Journal: Cells

Article Title: Correlation between Periostin Expression and Pro-Angiogenic Factors in Non-Small-Cell Lung Carcinoma

doi: 10.3390/cells13171406

Figure Lengend Snippet: Comparison of the expression of POSTN (in cells ( A ) and in stroma ( B )) and the expression of pro-angiogenic markers (VEGF-A ( C ); CD31 ( D ); CD34 ( E ); CD105 ( F )) between non-malignant lung tissue and cancer cells. The significance of the differences was determined using the Mann–Whitney U test. The images in ( G – K ) show punches from tissue microarrays illustrating an example of the immunohistochemical (IHC) reaction for each individual protein, as described above. Error bars represent the standard deviation (SD). *** p < 0.001.

Article Snippet: The following specific primary antibodies were used in the IHC experiments: anti-POSTN rabbit polyclonal antibody (1:200 dilution, NBP1-82472, Novus Biologicals, Littleton, CO, USA), monoclonal mouse anti-VEGF-A antibody (1:50 dilution + linker, VG1 clone, M7273, Agilent Technologies, Santa Clara, CA, USA), monoclonal mouse anti-CD31 antibody (RTU, JC70A clone, IR610, Agilent Technologies, Santa Clara, CA, USA), monoclonal mouse anti-CD34 antibody (RTU, QBEnd/10 clone, IR632, Agilent Technologies, Santa Clara, CA, USA), and rabbit polyclonal anti-CD105 antibody (1:1000 dilution, 10862-1-AP, Proteintech, Manchester, UK).

Techniques: Comparison, Expressing, MANN-WHITNEY, Immunohistochemical staining, Standard Deviation

Journal: Cells

Article Title: Correlation between Periostin Expression and Pro-Angiogenic Factors in Non-Small-Cell Lung Carcinoma

doi: 10.3390/cells13171406

Figure Lengend Snippet: Correlation plots comparing the quantities of CD31-positive ( A ), CD34-positive ( B ), and CD105-positive ( C ) vessels quantified via the Chalkley and Weidner methods. The plots include p -values and R-values (calculated with Spearman’s correlation test) and the number of cases, which can be seen in the lower right corner of each plot.

Article Snippet: The following specific primary antibodies were used in the IHC experiments: anti-POSTN rabbit polyclonal antibody (1:200 dilution, NBP1-82472, Novus Biologicals, Littleton, CO, USA), monoclonal mouse anti-VEGF-A antibody (1:50 dilution + linker, VG1 clone, M7273, Agilent Technologies, Santa Clara, CA, USA), monoclonal mouse anti-CD31 antibody (RTU, JC70A clone, IR610, Agilent Technologies, Santa Clara, CA, USA), monoclonal mouse anti-CD34 antibody (RTU, QBEnd/10 clone, IR632, Agilent Technologies, Santa Clara, CA, USA), and rabbit polyclonal anti-CD105 antibody (1:1000 dilution, 10862-1-AP, Proteintech, Manchester, UK).

Techniques:

Journal: Cells

Article Title: Correlation between Periostin Expression and Pro-Angiogenic Factors in Non-Small-Cell Lung Carcinoma

doi: 10.3390/cells13171406

Figure Lengend Snippet: Correlation plots comparing the expression of POSTN in stroma with the expression of pro-angiogenic markers, as quantified via the Weidner ( A – C ) or Chalkley ( D – F ) method. The pro-angiogenic factors are CD31 ( A , D ), CD34 ( B , E ), and CD105 ( C , F ). The plots include p -values and R-values (calculated with Spearman’s correlation test) and the number of cases, which can be seen in the lower right corner of each plot.

Article Snippet: The following specific primary antibodies were used in the IHC experiments: anti-POSTN rabbit polyclonal antibody (1:200 dilution, NBP1-82472, Novus Biologicals, Littleton, CO, USA), monoclonal mouse anti-VEGF-A antibody (1:50 dilution + linker, VG1 clone, M7273, Agilent Technologies, Santa Clara, CA, USA), monoclonal mouse anti-CD31 antibody (RTU, JC70A clone, IR610, Agilent Technologies, Santa Clara, CA, USA), monoclonal mouse anti-CD34 antibody (RTU, QBEnd/10 clone, IR632, Agilent Technologies, Santa Clara, CA, USA), and rabbit polyclonal anti-CD105 antibody (1:1000 dilution, 10862-1-AP, Proteintech, Manchester, UK).

Techniques: Expressing

Journal: Cells

Article Title: Correlation between Periostin Expression and Pro-Angiogenic Factors in Non-Small-Cell Lung Carcinoma

doi: 10.3390/cells13171406

Figure Lengend Snippet: Correlation plots comparing the expression of POSTN in cells ( A – C ) and in stroma ( D – F ) with the expression of pro-angiogenic markers, as quantified via the Chalkley method, assessed in adenocarcinoma. The pro-angiogenic factors are CD31 ( A , D ), CD34 ( B , E ), and CD105 ( C , F ). The plots include p -values and R-values (calculated with Spearman’s correlation test) and the number of cases, which can be seen in the lower right corner of each plot. The images in ( G – I ) show punches from tissue microarrays demonstrating an example of the immunohistochemical (IHC) reaction for each individual protein, as previously described. The images in ( J – L ) show representative magnified regions of the microarray punches.

Article Snippet: The following specific primary antibodies were used in the IHC experiments: anti-POSTN rabbit polyclonal antibody (1:200 dilution, NBP1-82472, Novus Biologicals, Littleton, CO, USA), monoclonal mouse anti-VEGF-A antibody (1:50 dilution + linker, VG1 clone, M7273, Agilent Technologies, Santa Clara, CA, USA), monoclonal mouse anti-CD31 antibody (RTU, JC70A clone, IR610, Agilent Technologies, Santa Clara, CA, USA), monoclonal mouse anti-CD34 antibody (RTU, QBEnd/10 clone, IR632, Agilent Technologies, Santa Clara, CA, USA), and rabbit polyclonal anti-CD105 antibody (1:1000 dilution, 10862-1-AP, Proteintech, Manchester, UK).

Techniques: Expressing, Immunohistochemical staining, Microarray

Journal: Cells

Article Title: Correlation between Periostin Expression and Pro-Angiogenic Factors in Non-Small-Cell Lung Carcinoma

doi: 10.3390/cells13171406

Figure Lengend Snippet: Correlation plots comparing the expression of POSTN in cells ( A – C ) and in stroma ( D – F ) with the expression of pro-angiogenic markers, as quantified via the Chalkley method, assessed in squamous-cell carcinoma. The pro-angiogenic factors are CD31 ( A , D ), CD34 ( B , E ), and CD105 ( C , F ). The plots include p -values and R-values (calculated with Spearman’s correlation test) and the number of cases, which can be seen in the lower right corner of each plot. The images in ( G – I ) show punches from tissue microarrays demonstrating an example of the immunohistochemical (IHC) staining for each individual protein, as previously described. The images in ( J – L ) show representative magnified regions of the microarray punches.

Article Snippet: The following specific primary antibodies were used in the IHC experiments: anti-POSTN rabbit polyclonal antibody (1:200 dilution, NBP1-82472, Novus Biologicals, Littleton, CO, USA), monoclonal mouse anti-VEGF-A antibody (1:50 dilution + linker, VG1 clone, M7273, Agilent Technologies, Santa Clara, CA, USA), monoclonal mouse anti-CD31 antibody (RTU, JC70A clone, IR610, Agilent Technologies, Santa Clara, CA, USA), monoclonal mouse anti-CD34 antibody (RTU, QBEnd/10 clone, IR632, Agilent Technologies, Santa Clara, CA, USA), and rabbit polyclonal anti-CD105 antibody (1:1000 dilution, 10862-1-AP, Proteintech, Manchester, UK).

Techniques: Expressing, Immunohistochemical staining, Immunohistochemistry, Microarray

Journal: iScience

Article Title: Patient-derived tumor organoids mimic treatment-induced DNA damage response in glioblastoma

doi: 10.1016/j.isci.2024.110604

Figure Lengend Snippet: Overview of the procedures used to generate GBOs from resected tumor tissue, cell-type-specific immunostaining of the GBOs and parental tumors and cytokine secretion from GBOs (A) Schematic presentation of main steps for generating GBOs from resected tumor tissue, created with bioRender.com . (B) Growth rate as the quantification of relative area of viable GBOs for five weeks. Data represent mean values ±S.E.M. ( n = 3 GBOs samples per patient (P)). (C) Immunofluorescence analyses of the GBO TME. GBOs express markers of GSCs (SOX2, CD9, and CD44) and TME cells, such as markers of cells of vasculature (CD31 and CD105), as well as macrophages and microglia (CD68 and Iba1). Representative images from a tissue sample from one patient are shown. Scale bar: 100 μm. (D) Selected cytokines and growth factors are secreted by GBOs in comparison to cultured GB cells from the same patient.

Article Snippet: Rabbit polyclonal to CD105 (ENG) , Atlas antibodies , Cat#HPA067440; RRID: AB_2685840.

Techniques: Immunostaining, Immunofluorescence, Comparison, Cell Culture

Journal: iScience

Article Title: Patient-derived tumor organoids mimic treatment-induced DNA damage response in glioblastoma

doi: 10.1016/j.isci.2024.110604

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal to CD105 (ENG) , Atlas antibodies , Cat#HPA067440; RRID: AB_2685840.

Techniques: Recombinant, Membrane, Blocking Assay, Red Blood Cell Lysis, Viability Assay, Reverse Transcription, Derivative Assay, Gene Expression, Software, Luminex

Journal: Cell Communication and Signaling : CCS

Article Title: Knockdown of trem2 promotes proinflammatory microglia and inhibits glioma progression via the JAK2/STAT3 and NF-κB pathways

doi: 10.1186/s12964-024-01642-6

Figure Lengend Snippet: Effect of trem2 on STAT1 phosphorylation and the NF-κB p50 signaling pathway, as well as animal studies. (a) Western blot analysis of itgam in HMC3 and BV2 cells. (b-c) Western blot analysis of anti-inflammatory cytokines (IL-10) and pro-inflammatory cytokines (IL-1β, IL-6, TNF-α). (d) Western blot analysis of NF-κB p50 in microglia. (e) Western blot analysis of jak2 and p-jak2 in microglia. (f) Western blot analysis of stat3 and p-stat3 in microglia. (g) Western blot analysis of stat1 and p-stat1 in microglia. (h) Representative image of intracranial tumor of mice ( n = 8 of each group). (i) The representative IHC images of IBA1. (j) The representative IF images of CD105. (k) The representative IF images of Ki-67. (l) The representative IHC images of γH 2 ax

Article Snippet: Primary antibodies included mouse anti-IBA1(1:400, Servicebio, Wuhan, GB12105), rabbit anti-trem2 (1:50, Thermofisher, USA, PA5-87933), rabbit anti-γH 2 ax (1:200, Abcam, ab81299), rabbit anti-CD105 (1:200, Bioss, bs-0597R), rabbit anti-Ki-67 (1:300, Affinity, AF0198).

Techniques: Western Blot

Journal: Bioengineering

Article Title: Evaluation of Skin Wound Healing with Biosheets Containing Somatic Stem Cells in a Dog Model: A Pilot Study

doi: 10.3390/bioengineering11050435

Figure Lengend Snippet: Macroscopic ( a ), H&E-stained ( b ), and Masson’s trichrome-stained ( c ) sections of biosheets formed in molds with different pore shapes. Collagen-rich tissue ( 1 ) and fibrin clot ( 2 ) positions formed in the molds with Y- and O/Y-shaped pores and their high-magnification images ( 1-1 , 1-2 ) and SSEA-4 and CD105 antigen-immunostained images ( 2-1 , 2-2 ).

Article Snippet: Subsequently, the sections were washed in distilled water twice for 10 min and blocked in 1% bovine serum albumin (Wako pure chemicals) in PBS at 24–26 °C for 1 h and incubated with anti-SSEA4 mouse monoclonal antibody (1:100, ab16287; Abcam, Cambridge, UK) and anti-CD105 rabbit polyclonal antibody (1:200, bs-0579R; Bioss Inc., Boston, MA, USA) overnight at 4 °C.

Techniques: Staining